Morphometrical and histological evaluation of the effect of vitamins B1, B6 and B12 on rats sciatic nerve after crush injury

Objectives: To evaluate the effect of vitamins (B1, B6, and B12) on nerve regeneration and recovery after crush injury to sciatic nerve in experimental rats Study Design: Prospective observational study Setting: Department of anatomy, College of medicine,Baghdad University Subjects and intervention: Total 60 Rats ,with ageing(8-12) weeks old of both sex, during 45 days period were included in this study Study period: 15 October 2016 to 30 November 2016 Results: on comparision among different groups ( control and experimental groups ) . Best improvement in group of rats on B12(group D) than other groups on B1(groupB) or B6(group C). B12 group treated with ( 1mg / kg / day ) significantly increased in the MTC when compared to the control , B6 and B1 groups on days 15, 30 and 45 of the study at P≤0.05. Histological analysis by Van Giesons stain,Aall groups control and experimental (B1,B6,B12,A) show degeneration in different areas but less in group B12. Bsciatic nerve fascicles with Schwann cell and fibroblast better organized in group B12 Conclusion: vitamin B12, among different kind of vitamins B, considered as a best vital medication for regeneration and remyelination of damage nerve fibers.